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                Mouse Anti-GSK-3 Beta antibody (bsm-33293M)
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                产品编号 bsm-33293M
                英文名称 GSK-3 Beta
                中文名称 糖原合酶激酶-3β单克隆抗体
                别    名 glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN.  
                研究领域 细胞生物  转录调节因子  激酶和磷酸酶  
                抗体来源 Mouse
                克隆类型 Monoclonal
                克 隆 号 3A6
                交叉反应 Human, Mouse, Rat, 
                产品应用 WB=1:500-1000 ELISA=1:1000-5000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-500 (石蜡切片需做抗原修★复)
                not yet tested in other applications.
                optimal dilutions/concentrations should be determined by the end user.
                分 子 量 47kDa
                细胞定位 细胞核 细胞浆 细胞膜 
                性    状 Lyophilized or Liquid
                浓    度 1mg/ml
                免 疫 原 KLH conjugated synthetic peptide derived from human GSK-3 Beta: 
                亚    型 IgG
                纯化方法 affinity purified by Protein G
                储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
                保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
                PubMed PubMed
                产品介绍 The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

                Function:
                Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.

                Subunit:
                Monomer. Interacts with ARRB2 and DISC1. Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

                Subcellular Location:
                Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

                Tissue Specificity:
                Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

                Post-translational modifications:
                Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216.

                Similarity:
                Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
                Contains 1 protein kinase domain.

                SWISS:
                P49841

                Gene ID:
                2932

                Database links:

                Entrez Gene: 2932 Human

                Entrez Gene: 56637 Mouse

                Entrez Gene: 84027 Rat

                Omim: 605004 Human

                SwissProt: P49841 Human

                SwissProt: Q9WV60 Mouse

                SwissProt: P18266 Rat

                Unigene: 445733 Human

                Unigene: 394930 Mouse

                Unigene: 10426 Rat



                Important Note:
                This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                产品图片
                Sample:
                A431 Cell (Human) Lysate at 40 ug
                A549 Cell (Human) Lysate at 40 ug
                DU145 Cell (Human) Lysate at 40 ug
                Primary: Anti- GSK-3 Beta (bsm-33293M) at 1/1000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 47 kD
                Observed band size: 47 kD
                Sample:
                Hela(Human) Cell Lysate at 30 ug
                Hela ko GSK-3 Beta (Human) Cell Lysate at 30 ug
                Primary: Anti-GSK-3 Beta (bsm-33293M) at 1/1000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 47 kD
                Observed band size: 47 kD
                Sample:
                Hela(Human) Cell Lysate at 30 ug
                293T(Human) Cell Lysate at 30 ug
                Primary: Anti-GSK-3 Beta (bsm-33293M) at 1/1000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 47 kD
                Observed band size: 48 kD
                Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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