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                Mouse Anti-Beta tubulin(Loading Control) antibody (bsm-33034M)
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                产品编号 bsm-33034M
                英文名称 Beta tubulin(Loading Control)
                中文名称 微管蛋白β tubulin/Tubulin β单克隆抗体(内参抗体)
                别    名 Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain.  
                产品类型 内参抗体 
                研究领域 细胞骨架  细胞外基质  
                抗体来源 Mouse
                克隆类型 Monoclonal
                克 隆 号 7G3
                交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, Monkey, Insect, Yeast
                产品应用 WB=1:1000-5000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-500 (石蜡切片需做抗原另外五大半神陡然凌空飞起修复)
                not yet tested in other applications.
                optimal dilutions/concentrations should be determined by the end user.
                分 子 量 55kDa
                细胞定位 细胞浆 
                性    状 Lyophilized or Liquid
                浓    度 1mg/ml
                免 疫 原 KLH conjugated synthetic peptide derived from human Beta tubulin: 
                亚    型 IgG1
                纯化方法 affinity purified by Protein G
                储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
                保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
                PubMed PubMed
                产品介绍 This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

                Function:
                Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

                Subunit:
                Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.

                Subcellular Location:
                Cytoplasmic, cytoskeleton.

                Tissue Specificity:
                Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

                Post-translational modifications:
                Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

                Similarity:
                Belongs to the tubulin family.

                SWISS:
                P07437

                Gene ID:
                203068

                Database links:

                Entrez Gene: 203068 Human

                Entrez Gene: 22154 Mouse

                Entrez Gene: 29214 Rat

                Omim: 191130 Human

                SwissProt: P07437 Human

                SwissProt: P99024 Mouse

                SwissProt: P69897 Rat

                Unigene: 636480 Human

                Unigene: 2458 Rat



                Important Note:
                This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                产品图片
                Sample:
                U87MG Cell (Human) Lysate at 40 ug
                SW480 Cell (Human) Lysate at 40 ug
                Primary: Anti- Beta tubulin (bsm-33034M) at 1/2 000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 55 kD
                Observed band size: 55 kD
                Sample:
                HL60(Human) Cell Lysate at 30 ug
                Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 55 kD
                Observed band size: 52 kD
                Sample:
                A431(Human) Cell Lysate at 30 ug
                SY5Y(Human) Cell Lysate at 30 ug
                Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 55 kD
                Observed band size: 52 kD
                Sample:
                HL60(Human) Cell Lysate at 30 ug
                U937(Human) Cell Lysate at 30 ug
                A431(Human) Cell Lysate at 30 ug
                SH-SY5Y(Human) Cell Lysate at 30 ug
                Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 55 kD
                Observed band size: 55 kD
                Sample:
                A431(Human) Cell Lysate at 30 ug
                Hela(Human) Cell Lysate at 30 ug
                Jurkat(Human) Cell Lysate at 30 ug
                Lovo(Human) Cell Lysate at 30 ug
                Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
                Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
                Predicted band size: 55 kD
                Observed band size: 52 kD
                Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
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